Mammalian, including human, spermatozoa undergo morphological and physiological changes during sperm maturation. Therefore, these changes may affect the fertilization and embryonic development. In this study, we examined the pronucleus formation,
pronucleus disappearance and embryonic development in the human oocytes fertilized by intracytoplasmic sperm injection (ICSI). The injected spermatozoa were grouped into ejaculated, epididymal and testicular by the collecting region. Among 363
metaphase
Ⅱ injected oocytes, 287 (79.1%) oocytes were normally fertilized and displayed two pronuclei. There were no difference in the fertilization rtes and in the pronucleus formation and pronucleus disappearance at 16, 20 and 24 hr after ICSI, among
the
each
spermatozoa group. Also, at 64 hr, the appearance of embryonic development was similar. From these results it can be concluded that there was no difference of maturity among the sperm collected from ejaculated, epididymis and testis in the
pronucleus
formation and embryonic development. Therefore, testicular spermatozoa are successfully used with ICSI in IVF-ET program.
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